Pascalito at 03:50, 13 October 2009

2009-10-13T03:50:36Z

Pascalito at 19:29, 28 September 2009

2009-09-28T19:29:25Z

Pascalito at 19:24, 28 September 2009

2009-09-28T19:24:24Z

Ariella at 16:53, 28 August 2009

2009-08-28T16:53:09Z

Ariella: New page: Aperio favors this sequence KOOORGGGGGGROOORGGGGGGGROGGGGGK While it's not conclusive, RGGGGGG has duped and we've seen GGGGGGR 3x and we've not seen a RGGGGGR yet. Ariella Solvent...

2009-08-14T00:35:01Z

New page: Aperio favors this sequence KOOORGGGGGGROOORGGGGGGGROGGGGGK While it's not conclusive, RGGGGGG has duped and we've seen GGGGGGR 3x and we've not seen a RGGGGGR yet. Ariella Solvent...

New page

Aperio favors this sequence

KOOORGGGGGGROOORGGGGGGGROGGGGGK

While it's not conclusive, RGGGGGG has duped and we've seen GGGGGGR 3x and we've not seen a RGGGGGR yet.

Ariella Solvent Data
RGGGGGG KOOORGG OGGGGGK OORGGGG GGGGGGR RGGGGGG OOORGGG
GGGGGRO GGGGROG GGGGGGG GGGROGG GROGGGG

KOOORGG
OOORGGG
OORGGGG
ORGGGGG
RGGGGGG
GGGGGGR
GGGGGRO
missing
missing
GGROOO
missing
ROOORG
OOORGGG
OORGGGG
RGGGGGG
GGGGGGG
GGGGGGR
GGGGGRO
GGGGROG
GGGROGG
missing
GROGGGG
OGGGGGK
KOOORGGGGGGROOORGGGGGGGROGGGGGK

Made two crosses of Amusement8G/Appreciation2Y KU1 to put a Y in the middle of the Gs on Amusement. Solvent data for that cross below.

GGGGGG OOORGG GGGGRO GGROOY

KU1 should hit 41.67% to 44.12% on a genome, this time a Y. Looks like GGROOY used to be GGROOO. On a 29 length genome the Y (or third O) would hit on the 12th/13th gene which would put 5 Gs between KOOOR and ROOOR.

KOOORGGGGGGGROGGGGGK Note: solvents below show different, elongated genome now.

Made two crosses putting a Y in gene 7 and 18 of Amusement, in the middle of the two long G sequences. Solvent data for that cross below. KOOORGGGyGGROOORGGGyGGGROGGGGGK - assumption

OORGGG - 4 GGGGGK ORGGGY GGGGRO - 2 OOORGG ROOORG GGROGG
RGGGYG GYGGGG RGGGYGG - 2 GGYGGGG - 2 GYGGGGR - 2

Aperio Solvent Data

OOORGGG
GGGGGGR x2
OGGGGGK
ROGGGGG
GGGGGRO x2
ROOORG

Aperio Solvent Data (200 Milky trials)

(45.5%) (91) GGG 1111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111
(04.0%) (08) GGK 11111111
(06.0%) (12) GGR 111111111111
(06.5%) (13) GRO 1111111111111
(03.5%) (07) KOO 1111111
(02.5%) (05) OGG 11111
(06.5%) (13) OOO 1111111111111
(06.0%) (12) OOR 111111111111
(08.0%) (16) ORG 1111111111111111
(06.5%) (13) RGG 1111111111111
(02.5%) (05) ROG 11111
(02.5%) (05) ROO 11111

Analysis (independent of the more complex solvents to validate the technique)

Assuming +/- 2.5% variance, all the non-GGG combinations fall within the same range with the marginal exception of ORG. Since both endpoints (which by definition will have 1 occurrence) are at the upper end of this range, I'm willing to expand the range to include ORG because we know the random number generation is weak and it's non-sensical to create a new bands of 2 occurrences to accommodate ORG when the endpoints would be present in that band.
GGG appears 9x as often as any other sequence, which suggests 11 Gs in a row OR two strings of Gs.
GGK terminates one of the GGG strings. GGR terminates another. It is possible that one or both could just be a double, but then we'd have to have repetitions to account for the GGG strings separately.
OGG begins a GGG string. RGG starts another. Two strings of GGG(x) and GGG(y) are conclusive. I'm using (x), (y), and (z) to indicate repetitions of the sequence. In some single gene sequences, I'm referring to the repetitions of the same letter in the gene to give its length and I need to adopt a clearer notations like [z] - Aperio

KOO 1
OOO 1
OOR 1
ORG 1
RGG 1

GGG(x)

GGR 1
GRO 1
ROG 1
OGG 1

GGG(y)

GGK

Combined with more complex clues

RGG starts no less than 6 Gs. This accounts for at least 4 of out repetitions.
OGG stats no more than 6 Gs because it's terminated by a K. This accounts for 3 of our 9 repetitions.
We have a GGGGGGG[7] clue, so we know that RGG must lead to at least 5 of our repetitions.
Since we have no expectation of a 3rd string of GGG(z), we must assume that RGG starts a sequence of 8 Gs to permit 6 repetitions.
[POST-MORTEM] I have missed ROO in this analysis.

KOOORGGGGGGGGROGGGGGK
KOO 1
OOO 1
OOR 1
ORG 1
RGG 1
KOOORGG CONFIRMED
GGGGGGGG GGG(x=8)
OOORGGG CONFIRMED
OORGGGG CONFIRMED
RGGGGGG CONFIRMED
GGGGGGG LIKELY
GGGGGGG LIKELY(2)
GGR 1
GGGGGGR CONFIRMED
GRO 1
GGGGGRO CONFIRMED
ROG 1
GGGGROG CONFIRMED
OGG 1
GGGROGG CONFIRMED
GGGGG GGG(y=5)
GROGGGG CONFIRMED
OGGGGGK CONFIRMED
GGK
KOOORGGGGGGGGROGGGGGK

This is really tight, but ROOORG from my later Crystal trial is UNACCOUNTED FOR - Aperio
[POST-MORTEM] I still have missed ROO in this analysis.
This suggests another string of OOO to account for ROOORG.
OOR density supports this string. ROO density does not. ORG density DEFINITELY supports this but was overlooked intentionally.
This implies another RGG string which is supported, also.
If 8% is the 2nd band, then 44% is 11 repeats of GGG and 28% is 12 repeats
if 7.5% is the 2nd band, then 45% is 12 repeats. I lean towards 12 here.

11-12 GGG 1111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111
1 GGK 11111111
2 GGR 111111111111
2 GRO 1111111111111
1 KOO 1111111
1 OGG 11111
2 OOO 1111111111111
2 OOR 111111111111
2 ORG 1111111111111111
2 RGG 1111111111111
1 ROG 11111
1 ROO 11111

KOOORGGGGGGROOORGGGGGGGROGGGGGK
KOOORGG
KOO 1
OOO 1
OOR 1
ORG 1
RGG 1
KOOORGGGGGGROOORGGGGGGGROGGGGGK
OORGGGG
KOOORGGGGGGROOORGGGGGGGROGGGGGK
RGGGGGG
GGG 1
GGG 2
GGG 3
GGG 4
KOOORGGGGGGROOORGGGGGGGROGGGGGK
GGGGGGR
GGR 1
KOOORGGGGGGROOORGGGGGGGROGGGGGK
GGGGGRO
GRO 1
KOOORGGGGGGROOORGGGGGGGROGGGGGK
ROOORG
ROO 1
OOO 2
OOR 2
ORG 2
KOOORGGGGGGROOORGGGGGGGROGGGGGK
OORGGGG
RGG 2
???????
KOOORGGGGGGROOORGGGGGGGROGGGGGK
RGGGGGG
GGG 5
GGG 6
GGG 7
GGG 8
KOOORGGGGGGROOORGGGGGGGROGGGGGK
GGGGGGG (GGGx5)
GGG 9
KOOORGGGGGGROOORGGGGGGGROGGGGGK
GGGGGGR
GGR 2
KOOORGGGGGGROOORGGGGGGGROGGGGGK
GGGGGRO
GRO 2
KOOORGGGGGGROOORGGGGGGGROGGGGGK
GGGGROG
ROG 1
KOOORGGGGGGROOORGGGGGGGROGGGGGK
GGGROGG
OGG 1
KOOORGGGGGGROOORGGGGGGGROGGGGGK
GROGGGG
GGG 10
GGG 11
???????
KOOORGGGGGGROOORGGGGGGGROGGGGGK
OGGGGGK (GGGx3)
GGG 12
GGK 1
KOOORGGGGGGROOORGGGGGGGROGGGGGK

@@ Line 1: / Line 1: @@
 KOOORGGG?GGGROOORGGG?GGGROGGGGGK
 length is 33, G-groups are longer, 5 total G's to be inserted in ?
 Ariella Solvent Data

← Older revision		Revision as of 19:29, 28 September 2009
Line 1:		Line 1:
−	~~KOOORGGGGGGROOORGGGGGGGROGGGGGK~~	+	KOOORGGG?GGGROOORGGG?GGGROGGGGGK
		+	length is 33, G-groups are longer, 5 total G's to be inserted in ?

	Ariella Solvent Data		Ariella Solvent Data

@@ Line 3: / Line 3: @@
 Ariella Solvent Data
 RGGGGGG  KOOORGG  OGGGGGK  OORGGGG  GGGGGGR  RGGGGGG  OOORGGG
 GGGGGRO  GGGGROG  GGGGGGG  GGGROGG  GROGGGG

@@ Line 1: / Line 1: @@
-Aperio favors this sequence
+KOOORGGGGGGROOORGGGGGGGROGGGGGK
 Ariella Solvent Data
@@ Line 18: / Line 12: @@
      ORGGGGG
       RGGGGGG
-       GGGGGGR
-        GGGGGRO
+       GGGGGGG
-         missing
+        GGGGGGR
           missing
-           GGROOO
+           missing
-           missing
+           GGROOO
-            ROOORG
+            missing
-             OOORGGG
+             ROOORG
-              OORGGGG
+              OOORGGG
-                RGGGGGG
+               OORGGGG
-                 GGGGGGG
+                 RGGGGGG
-                  GGGGGGR
-                   GGGGGRO
+                  GGGGGGG
-                    GGGGROG
+                   GGGGGGR
-                     GGGROGG
+                    GGGGGRO
-                      missing
+                     GGGGROG
-                       GROGGGG
+                      GGGROGG
-                         OGGGGGK
+                       missing
-  KOOORGGGGGGROOORGGGGGGGROGGGGGK
+                        GROGGGG
 Made two crosses of Amusement8G/Appreciation2Y KU1 to put a Y in the middle of the Gs on Amusement. Solvent data for that cross below.

Amusement Data - Revision history

Pascalito at 03:50, 13 October 2009

Pascalito at 19:29, 28 September 2009

Pascalito at 19:24, 28 September 2009

Ariella at 16:53, 28 August 2009

Ariella: New page: Aperio favors this sequence KOOORGGGGGGROOORGGGGGGGROGGGGGK While it's not conclusive, RGGGGGG has duped and we've seen GGGGGGR 3x and we've not seen a RGGGGGR yet. Ariella Solvent...