OE01 - Revision history

GHawkins: /* Eleventh mutation test: L-Crimson R-Crimson */

2012-09-19T00:39:44Z

Eleventh mutation test: L-Crimson R-Crimson

GHawkins: /* Eleventh mutation test: L-Crimson R-Crimson */

2012-09-19T00:35:31Z

Eleventh mutation test: L-Crimson R-Crimson

GHawkins: /* Eleventh mutation test: L-Crimson R-Crimson */

2012-09-19T00:35:19Z

Eleventh mutation test: L-Crimson R-Crimson

GHawkins at 16:37, 18 September 2012

2012-09-18T16:37:53Z

GHawkins: /* Eight mutation test: L-Dusk R-Morning */

2012-09-17T17:53:38Z

Eight mutation test: L-Dusk R-Morning

GHawkins: /* Next Step */

2012-09-17T17:49:45Z

Next Step

GHawkins at 17:33, 17 September 2012

2012-09-17T17:33:13Z

GHawkins at 01:28, 17 September 2012

2012-09-17T01:28:47Z

GHawkins at 00:51, 17 September 2012

2012-09-17T00:51:50Z

GHawkins at 23:17, 16 September 2012

2012-09-16T23:17:54Z

@@ Line 110: / Line 110: @@
 The Crimson lily genome has recently been approved for testing, so this test will be a quick check on whether a mutation on two of those lilies can be readily interpreted.
-Left splint should hit gene 33 or 34
+Left splint should hit gene 33 or 34<br>
-Right splint should hit gene 25
+Right splint should hit gene 25<br>
-Genes 23-34: IY IY UOOO UYYY
+Genes 23-34: IY IY UOOO UYYY<br>
 On the left, an Inner East has lost some Yellow color, but the stamen remains unchanged.  This is consistent with the third Y of UYYY being replaced with an I.

@@ Line 119: / Line 119: @@
 Both tests seem to confirm:<br>
-Left-Crimson 34(Y)
+Left-Crimson 34(Y)<br>
 Right-Crimson 25(I)

@@ Line 109: / Line 109: @@
 =Eleventh mutation test:  L-Crimson R-Crimson=
 The Crimson lily genome has recently been approved for testing, so this test will be a quick check on whether a mutation on two of those lilies can be readily interpreted.
 =References=

← Older revision		Revision as of 16:37, 18 September 2012
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	Magenta has been removed from the Delicate outer stamen and Yellow is visible on the entire stamen of Energy. Left-Delicate 5(I) Right-Energy 10(U)		Magenta has been removed from the Delicate outer stamen and Yellow is visible on the entire stamen of Energy. Left-Delicate 5(I) Right-Energy 10(U)
		+
		+	=Eleventh mutation test: L-Crimson R-Crimson=
		+	The Crimson lily genome has recently been approved for testing, so this test will be a quick check on whether a mutation on two of those lilies can be readily interpreted.

	=References=		=References=

← Older revision		Revision as of 17:53, 17 September 2012
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	Outer stamen only: Right-Morning 11 (Y)		Outer stamen only: Right-Morning 11 (Y)
		+
		+	Additionally, this result eliminates Right-Silken 4(O) as a possibility, leaving us with Right-Silken 5(O)

	=Ninth mutation test: L-Energy R-Delicate=		=Ninth mutation test: L-Energy R-Delicate=

← Older revision		Revision as of 17:49, 17 September 2012
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	The fourth test allows us to narrow down the results of the third test. The range of the left splint has been reduced to 11-13 on Clarity, so 10(Y) has been eliminated. The range of the right splint has been reduced to 9-11. This indicates that gene 8 was NOT affected on the crown, so Clarity 11(I) has been eliminated. Of genes 9-11 on Crown, only gene 9 (U) could replace that Y without having a visible effect on the Stamen. Hence left-Clarity 12(Y) Right-Crown 9(U). Since the gene sizes of Clarity and Crown are nearly identical, we would expect that the left and right genes hit would remain the same when we swap Clarity and Crown, which is exactly what is observed.		The fourth test allows us to narrow down the results of the third test. The range of the left splint has been reduced to 11-13 on Clarity, so 10(Y) has been eliminated. The range of the right splint has been reduced to 9-11. This indicates that gene 8 was NOT affected on the crown, so Clarity 11(I) has been eliminated. Of genes 9-11 on Crown, only gene 9 (U) could replace that Y without having a visible effect on the Stamen. Hence left-Clarity 12(Y) Right-Crown 9(U). Since the gene sizes of Clarity and Crown are nearly identical, we would expect that the left and right genes hit would remain the same when we swap Clarity and Crown, which is exactly what is observed.
−
−	~~Additionally, this range eliminates gene 5 of Silken as a possibility, so Right-Silken 4(O).~~

	Can we narrow it down to a single gene on Dusk yet? On the left splint, the range hit is anywhere from genes 23-26. On the right splint, the range hit is anywhere from genes 17-20.		Can we narrow it down to a single gene on Dusk yet? On the left splint, the range hit is anywhere from genes 23-26. On the right splint, the range hit is anywhere from genes 17-20.

@@ Line 81: / Line 81: @@
 For Morning, on the left splint the toxin will mutate either the 14th or 15th gene.  On the right splint, it will mutate the 10th or 11th gene.  All of these genes are a Y!
-The first  genes of Delicate:  IOIOIO
+The first 6 genes of Delicate:  IOIOIO
 The first 17 genes of Energy:  VVVVVUYYYUYYYGYYY
@@ Line 100: / Line 100: @@
 =Ninth mutation test:  L-Energy R-Delicate=
 =Tenth mutation test:  L-Delicate R-Energy=
 =References=

@@ Line 92: / Line 92: @@
 =Seventh mutation test:  L-Morning R-Dusk=
 =Eight mutation test:  L-Dusk R-Morning=
 =Ninth mutation test:  L-Energy R-Delicate=

← Older revision		Revision as of 00:51, 17 September 2012
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	Now that we know an I has been transferred to Blush and replaced an O, but the stamen hasn't changed at all. Therefore it must have been the last O of the disrupted GOOO allele. Left-Blush 9(O).		Now that we know an I has been transferred to Blush and replaced an O, but the stamen hasn't changed at all. Therefore it must have been the last O of the disrupted GOOO allele. Left-Blush 9(O).
		+
		+	=Final Tests=
		+	That leaves us with Delicate (16), Energy (42), Fracture (18), and Morning (44) to characterize. Testing Fracture isn't strictly necessary since it has a gene length identical to Silken, so we know exactly where the toxin will mutate that lily.
		+
		+	For Delicate, on the left split the toxin will mutate either the 5th or 6th gene. On the right split, it will mutate the 4th gene.
		+
		+	For Energy, on the left splint the toxin will mutate either the 13th or 14th gene. On the right splint, it will mutate the 9th or 10th gene.
		+
		+	For Morning, on the left splint the toxin will mutate either the 14th or 15th gene. On the right splint, it will mutate the 10th or 11th gene. All of these genes are a Y!
		+
		+	The first genes of Delicate: IOIOIO
		+
		+	The first 17 genes of Energy: VVVVVUYYYUYYYGYYY
		+
		+	The first 16 genes of Morning: IRRRIYYYIYYYUYYY
		+
		+	For Morning, an I would be ideal, which would work with a Dusk double cross since the donated I will allow us to see exactly which gene was hit on both the left and right cross.
		+
		+	On Energy, the difference between a mutation on the 13th and 14th gene is clear based on which petal set is affected. Double crossing with Delicate would likely be informative.
		+
		+	=Seventh mutation test: L-Morning R-Dusk=
		+
		+	=Eight mutation test: L-Dusk R-Morning=
		+
		+	=Ninth mutation test: L-Energy R-Delicate=
		+
		+	=Tenth mutation test: L-Delicate R-Energy=

	=References=		=References=

@@ Line 61: / Line 61: @@
 Genes R and O would have a uniquely identifiable effect in genes 23-26.
-On Blush, the left splint would affect 6-8, right would hit 8-10.  GOOOV  This could be informative, let's get to it.
+On Blush, the left splint would affect 6-8, right would hit 8-10.  Genes 6-10 are GOOOV  This could be informative, let's get to it.
 =Fifth mutation test:  L-Dusk R-Blush=
 =Sixth mutation test:  L-Blush R-Dusk=
 =References=