OE07 - Revision history

GHawkins at 02:50, 4 October 2012

2012-10-04T02:50:45Z

GHawkins at 05:44, 3 October 2012

2012-10-03T05:44:28Z

GHawkins at 05:35, 3 October 2012

2012-10-03T05:35:52Z

GHawkins at 05:34, 3 October 2012

2012-10-03T05:34:11Z

GHawkins: /* Sixth mutation test: L-Vampire R-Dusk */

2012-10-03T04:18:15Z

Sixth mutation test: L-Vampire R-Dusk

GHawkins: /* Discussion and next step */

2012-10-03T04:17:34Z

Discussion and next step

GHawkins at 04:17, 3 October 2012

2012-10-03T04:17:07Z

GHawkins: /* Seventh mutation test: L-Crimson R-Crimson */

2012-09-28T07:25:58Z

Seventh mutation test: L-Crimson R-Crimson

GHawkins: /* Fourth mutation test: L-Fracture R-Delicate */

2012-09-28T07:24:23Z

Fourth mutation test: L-Fracture R-Delicate

GHawkins at 06:36, 28 September 2012

2012-09-28T06:36:59Z

@@ Line 102: / Line 102: @@
 =Eighth mutation test: L-Crimson R-Crimson=
 Both lilies were unchanged.  This can only have happened if gene 83 replaced gene 51.
 =CUTOFF=
 Progress has been made on narrowing the splint ranges.  Back to Crimson:

@@ Line 97: / Line 97: @@
 =Discussion and next step=
 Progress has been made on narrowing the splint ranges.  Back to Crimson:
-Crimson (Starting from gene 47):  URO'''R G'''OOO GOOO GOOO GYYY GYYY GYYY GROR GROR GROR GROR GR'''RO''' GRRO GRRO GRRO<br>
+Crimson (Starting from gene 47):  U'''ROR GOO'''O GOOO GOOO GYYY GYYY GYYY GROR GROR '''G'''ROR GROR GRRO GRRO GRRO GRRO<br>
-Left splint:  50-51<br>
+Left splint:  48-53<br>
-Right splint:  93-94<br>
+Right splint:  83<br>
 That's much easier to work with, we can even cross Crimson with itself and know at least what gene was affected on the left splint.

@@ Line 90: / Line 90: @@
 Left-Dusk Right-Energy might work.
-=Sixth mutation test:  L-Dusk R-Energy=
+=Seventh mutation test:  L-Dusk R-Energy=
 Dusk has lost North-Magenta, Energy has lost West-Yellow<br>
-Left-Dusk 35(G)<br>
+Left-Dusk 35(G) or 37(O)<br>
-Right-Energy 37(R)
+Right-Energy 33(R)
 =Discussion and next step=

@@ Line 75: / Line 75: @@
 =Sixth mutation test:  L-Vampire R-Dusk=
-=CUTOFF=
+Right-Dusk 57 (Y)
-Crimson (Starting from gene 47):  UR'''OR GOO'''O GOOO GOOO GYYY GYYY GYYY GROR GROR '''GROR G'''ROR GRRO GRRO GRRO GRRO<br>
+=Discussion and next step=
-Energy:  Energy VVVVV UYYY UYYY GYYY GY'''YY''' GRRO GRRO URO'''R UR'''OR VVVVV<br>
+Now for the left splint of dusk
-Left splint:  20-21<br>
-Right splint:  33-35<br>
+Dusk:  GROR GORR UYYY GYOR IY GRRO IYYY GRRO GOR'''R GRO'''R ROYG IO GROR IOOO IYY I'''Y URR'''R GRRO GORR UOOO<br>
 Left-Dusk Right-Energy might work.

@@ Line 76: / Line 76: @@
 =Sixth mutation test:  L-Vampire R-Dusk=
+=CUTOFF=
 Crimson (Starting from gene 47):  UR'''OR GOO'''O GOOO GOOO GYYY GYYY GYYY GROR GROR '''GROR G'''ROR GRRO GRRO GRRO GRRO<br>

@@ Line 67: / Line 67: @@
 =Discussion and next step=
-With the range now significantly narrowed, can we work with Dusk or Crimson?
+Can we work with Dusk or Crimson?
 Dusk:  GROR GORR UYYY GYOR IY GRRO IYYY GRRO GOR'''R GRO'''R ROYG IO GROR IOOO IYY I'''Y URR'''R GRRO GORR UOOO<br>
@@ Line 74: / Line 74: @@
 Putting an O onto the right splint would be oddly informative.
-=Sixth mutation test:  L-Vampire R-Dusk
+=Sixth mutation test:  L-Vampire R-Dusk=

@@ Line 31: / Line 31: @@
 =Discussion and next step=
-Knowing that the right splint hits so far to the right, and a Y is taken from Silken on the left splint tells us that an I must have been removed from Vampire.
+Knowing that the right splint hits so far to the right, and a Y is taken from Silken on the left splint tells us that it was the O from gene 10 or 12 from Vampire that was removed.
-Right-Vampire 11(I)
+Right-Vampire 10 or 12(O)
-That restricts the range on Delicate to either gene 14 (Y) or 15 (O).  Since a Y was taken from Fracture on the left splint, that leaves us with:
+The range on Silken is now from 14-18.  However, 17 and 18 can be eliminated since otherwise color would have been visible on Silken's stamen in the second test.
-Right-Delicate 15(O)
+Given the effects on fracture, the genes can be further narrowed to 15 and 16.
-Which narrows us down to either gene 16 or 17 on Fracture and Silken.
+On Delicate, since we know that a Y which followed I was transferred and color in the outer stamen was lost but not replaced, it must be gene 15.
 Where does that lead us next?
-Morning:  IRRR IYYY IYYY UYYY UYY'''Y UR'''OR UROR GRRR GYYY GO'''RR G'''RRO <br>
+Morning:  IRRR IYYY IYYY UYYY UYY'''Y UR'''OR UROR GRRR GY'''YY''' GORR GRRO <br>
 Left Splint:  20-22<br>
-Right Splint:  39-41<br>
+Right Splint:  35-36<br>
-That's a fairly useful range, even with many possible different genes, we can generally readily establish which petal was affected which helps us narrow down gene ranges quite well.
+That's a fairly difficult range.
-Clarity:  IRIR IOIO IYIY GORR '''GR'''OR GRRO GORR GRO'''R G'''RRO <br>
+Clarity:  IRIR IOIO IYIY GORR '''GR'''OR GRRO GORR '''GR'''OR GRRO <br>
 Left Splint:  17-18<br>
-Right Splint:  32-33<br>
+Right Splint:  29-30<br>
-Left-Morning Right-Clarity looks very informative.  Every possible result has a unique interpretation.<br>
+Left-Morning Right-Clarity looks very informative.<br>
 Left-Clarity Right-Morning looks much less useful, so we'll stick to one test for now.
 =Fifth mutation test:  L-Morning R-Clarity=
 Morning has lost Yellow-West, Clarity has lost Magenta-North.<br>
-Left-Morning 21(U)<br>
-Right-Clarity 32(R)
+Either the R and G were swapped, or the U and R were swapped.
 =Discussion and next step=
 With the range now significantly narrowed, can we work with Dusk or Crimson?
-Dusk:  GROR GORR UYYY GYOR IY GRRO IYYY GRRO GOR'''R G'''ROR ROYG IO GROR IOOO IYY IY URRR GR'''RO''' GORR UOOO<br>
+Dusk:  GROR GORR UYYY GYOR IY GRRO IYYY GRRO GOR'''R GRO'''R ROYG IO GROR IOOO IYY I'''Y URR'''R GRRO GORR UOOO<br>
-Left splint:  34-35<br>
+Left splint:  34-37<br>
-Right splint:  64-65<br>
+Right splint:  57-60<br>
-The left splint should be easy to test.
+Putting an O onto the right splint would be oddly informative.
-Crimson (Starting from gene 47):  UR'''OR G'''OOO GOOO GOOO GYYY GYYY GYYY GROR GROR GROR GROR GR'''RO G'''RRO GRRO GRRO<br>
+Crimson (Starting from gene 47):  UR'''OR GOO'''O GOOO GOOO GYYY GYYY GYYY GROR GROR '''GROR G'''ROR GRRO GRRO GRRO GRRO<br>
-Left splint:  49-51<br>
+Left splint:  49-53<br>
-Right splint:  93-95<br>
+Right splint:  83-87<br>
 Not very promising.
-Energy:  Energy VVVVV UYYY UYYY GYYY GY'''Y'''Y GRRO GRRO UROR URO'''R V'''VVVV<br>
+Energy:  Energy VVVVV UYYY UYYY GYYY GY'''YY''' GRRO GRRO URO'''R UR'''OR VVVVV<br>
-Left splint:  20<br>
+Left splint:  20-21<br>
-Right splint:  37-38<br>
+Right splint:  33-35<br>
-Left-Dusk Right-Energy would work just fine.
+Left-Dusk Right-Energy might work.
 =Sixth mutation test:  L-Dusk R-Energy=

@@ Line 92: / Line 92: @@
 =Seventh mutation test:  L-Crimson R-Crimson=
-No change to either flower.
+No change to either flower.<br>
-Left-Crimson 50 R
+Left-Crimson 50 R<br>
 Right-Crimson 93 R

@@ Line 24: / Line 24: @@
 Fracture is now double giant, and Delicate is normal size.  We know from previous tests that on the left splint, the gene affected is left of center.  Since the giant was disrupted but not one of the dwarf alleles, we know that gene 9 must have been affected.  This allows us to identify the left gene affected on all lilies.
-Left-Fracture 9(Y)
+Left-Fracture 9(Y)<br>
-Left-Silken 9(Y)
+Left-Silken 9(Y)<br>
-Left-Vampire 6(O)
+Left-Vampire 6(O)<br>
-Left-Delicate 8(Y)
+Left-Delicate 8(Y)<br>
 =Discussion and next step=