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Difference between revisions of "Amusement Data"

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KOOORGGGGGGROOORGGGGGGGROGGGGGK
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KOOORGGG?GGGROOORGGG?GGGROGGGGGK
 +
length is 33, G-groups are longer, 5 total G's to be inserted in ?
 +
 
 +
Mutagen results show it '''cannot be length 33''', then 15th would be O and 16th G
  
 
Ariella Solvent Data
 
Ariella Solvent Data
 
RGGGGGG  KOOORGG  OGGGGGK  OORGGGG  GGGGGGR  RGGGGGG  OOORGGG   
 
RGGGGGG  KOOORGG  OGGGGGK  OORGGGG  GGGGGGR  RGGGGGG  OOORGGG   
GGGGGRO  GGGGROG  GGGGGGG  GGGROGG  GROGGGG
+
GGGGGRO  GGGGROG  GGGGGGG  GGGROGG  GROGGGG  
 +
 
 +
after changing 8 or 9th gene to a Y : KOOORGGGggGR...; solvent results GYGG YGGG means at least 1 extra G in first G=group
  
  

Latest revision as of 03:50, 13 October 2009

KOOORGGG?GGGROOORGGG?GGGROGGGGGK length is 33, G-groups are longer, 5 total G's to be inserted in ?

Mutagen results show it cannot be length 33, then 15th would be O and 16th G

Ariella Solvent Data RGGGGGG KOOORGG OGGGGGK OORGGGG GGGGGGR RGGGGGG OOORGGG GGGGGRO GGGGROG GGGGGGG GGGROGG GROGGGG

after changing 8 or 9th gene to a Y : KOOORGGGggGR...; solvent results GYGG YGGG means at least 1 extra G in first G=group


KOOORGG
 OOORGGG
  OORGGGG
   ORGGGGG
    RGGGGGG
       ?
     GGGGGGG
      GGGGGGR
       GGGGGRO
        missing
         missing
          GGROOO
           missing
            ROOORG
             OOORGGG
              OORGGGG
                RGGGGGG
                  ?
                 GGGGGGG
                  GGGGGGR
                   GGGGGRO
                    GGGGROG
                     GGGROGG
                      missing
                       GROGGGG
                         OGGGGGK
KOOORGGGGGGGROOORGGGGGGGROGGGGGK  Three missing Gs, could be 6 Gs in one of the Gstrings

Made two crosses of Amusement8G/Appreciation2Y KU1 to put a Y in the middle of the Gs on Amusement. Solvent data for that cross below.

GGGGGG OOORGG GGGGRO GGROOY


KU1 should hit 41.67% to 44.12% on a genome, this time a Y. Looks like GGROOY used to be GGROOO. On a 29 length genome the Y (or third O) would hit on the 12th/13th gene which would put 5 Gs between KOOOR and ROOOR.


KOOORGGGGGGGROGGGGGK  Note: solvents below show different, elongated genome now.

Made two crosses putting a Y in gene 7 and 18 of Amusement, in the middle of the two long G sequences. Solvent data for that cross below. KOOORGGGyGGROOORGGGyGGGROGGGGGK - assumption

OORGGG - 4 GGGGGK ORGGGY GGGGRO - 2 OOORGG ROOORG GGROGG RGGGYG GYGGGG RGGGYGG - 2 GGYGGGG - 2 GYGGGGR - 2


Aperio Solvent Data

OOORGGG
GGGGGGR x2
OGGGGGK
ROGGGGG
GGGGGRO x2
ROOORG


Aperio Solvent Data (200 Milky trials)

(45.5%) (91) GGG 1111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111
(04.0%) (08) GGK 11111111
(06.0%) (12) GGR 111111111111
(06.5%) (13) GRO 1111111111111
(03.5%) (07) KOO 1111111
(02.5%) (05) OGG 11111
(06.5%) (13) OOO 1111111111111
(06.0%) (12) OOR 111111111111
(08.0%) (16) ORG 1111111111111111
(06.5%) (13) RGG 1111111111111
(02.5%) (05) ROG 11111
(02.5%) (05) ROO 11111

Analysis (independent of the more complex solvents to validate the technique)

Assuming +/- 2.5% variance, all the non-GGG combinations fall within the same range with the marginal exception of ORG. Since both endpoints (which by definition will have 1 occurrence) are at the upper end of this range, I'm willing to expand the range to include ORG because we know the random number generation is weak and it's non-sensical to create a new bands of 2 occurrences to accommodate ORG when the endpoints would be present in that band. GGG appears 9x as often as any other sequence, which suggests 11 Gs in a row OR two strings of Gs. GGK terminates one of the GGG strings. GGR terminates another. It is possible that one or both could just be a double, but then we'd have to have repetitions to account for the GGG strings separately. OGG begins a GGG string. RGG starts another. Two strings of GGG(x) and GGG(y) are conclusive. I'm using (x), (y), and (z) to indicate repetitions of the sequence. In some single gene sequences, I'm referring to the repetitions of the same letter in the gene to give its length and I need to adopt a clearer notations like [z] - Aperio

KOO 1
 OOO 1
  OOR 1
   ORG 1
    RGG 1


      GGG(x) 


         GGR 1
          GRO 1
           ROG 1
            OGG 1


              GGG(y)


                  GGK

Combined with more complex clues

RGG starts no less than 6 Gs. This accounts for at least 4 of out repetitions. OGG stats no more than 6 Gs because it's terminated by a K. This accounts for 3 of our 9 repetitions. We have a GGGGGGG[7] clue, so we know that RGG must lead to at least 5 of our repetitions. Since we have no expectation of a 3rd string of GGG(z), we must assume that RGG starts a sequence of 8 Gs to permit 6 repetitions. [POST-MORTEM] I have missed ROO in this analysis.

KOOORGGGGGGGGROGGGGGK
KOO 1
 OOO 1
  OOR 1
   ORG 1
    RGG 1
KOOORGG CONFIRMED
     GGGGGGGG GGG(x=8)
 OOORGGG CONFIRMED
  OORGGGG CONFIRMED
    RGGGGGG CONFIRMED
     GGGGGGG LIKELY
      GGGGGGG LIKELY(2)
           GGR 1
       GGGGGGR CONFIRMED
            GRO 1
        GGGGGRO CONFIRMED
             ROG 1
         GGGGROG CONFIRMED
              OGG 1
          GGGROGG CONFIRMED
               GGGGG GGG(y=5)
            GROGGGG CONFIRMED
              OGGGGGK CONFIRMED
                  GGK
KOOORGGGGGGGGROGGGGGK


This is really tight, but ROOORG from my later Crystal trial is UNACCOUNTED FOR - Aperio [POST-MORTEM] I still have missed ROO in this analysis. This suggests another string of OOO to account for ROOORG. OOR density supports this string. ROO density does not. ORG density DEFINITELY supports this but was overlooked intentionally. This implies another RGG string which is supported, also. If 8% is the 2nd band, then 44% is 11 repeats of GGG and 28% is 12 repeats if 7.5% is the 2nd band, then 45% is 12 repeats. I lean towards 12 here.

11-12 GGG 1111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111
1 GGK 11111111
2 GGR 111111111111
2 GRO 1111111111111
1 KOO 1111111
1 OGG 11111
2 OOO 1111111111111
2 OOR 111111111111
2 ORG 1111111111111111
2 RGG 1111111111111
1 ROG 11111
1 ROO 11111


KOOORGGGGGGROOORGGGGGGGROGGGGGK
KOOORGG
KOO 1
 OOO 1
  OOR 1
   ORG 1
    RGG 1
KOOORGGGGGGROOORGGGGGGGROGGGGGK
  OORGGGG
KOOORGGGGGGROOORGGGGGGGROGGGGGK
    RGGGGGG
     GGG 1
      GGG 2
       GGG 3
        GGG 4
KOOORGGGGGGROOORGGGGGGGROGGGGGK
     GGGGGGR
         GGR 1
KOOORGGGGGGROOORGGGGGGGROGGGGGK
      GGGGGRO
          GRO 1
KOOORGGGGGGROOORGGGGGGGROGGGGGK
           ROOORG
           ROO 1
            OOO 2
             OOR 2
              ORG 2
KOOORGGGGGGROOORGGGGGGGROGGGGGK
             OORGGGG
               RGG 2
              ???????
KOOORGGGGGGROOORGGGGGGGROGGGGGK
               RGGGGGG
                GGG 5
                 GGG 6
                  GGG 7
                   GGG 8
KOOORGGGGGGROOORGGGGGGGROGGGGGK
                GGGGGGG (GGGx5)
                    GGG 9
KOOORGGGGGGROOORGGGGGGGROGGGGGK
                 GGGGGGR
                     GGR 2
KOOORGGGGGGROOORGGGGGGGROGGGGGK
                  GGGGGRO
                      GRO 2
KOOORGGGGGGROOORGGGGGGGROGGGGGK
                   GGGGROG
                       ROG 1
KOOORGGGGGGROOORGGGGGGGROGGGGGK
                    GGGROGG
                        OGG 1
KOOORGGGGGGROOORGGGGGGGROGGGGGK
                      GROGGGG
                         GGG 10
                          GGG 11
                       ???????
KOOORGGGGGGROOORGGGGGGGROGGGGGK
                        OGGGGGK (GGGx3)
                           GGG 12
                            GGK 1
KOOORGGGGGGROOORGGGGGGGROGGGGGK