|
|
| (194 intermediate revisions by the same user not shown) |
| Line 3: |
Line 3: |
| | ''' | | ''' |
| | | | |
| − | Originally from the Bastet server, I transfered to Tale 5 only a few months ago with some friends. (Now we form the Bastet Hounds Guild). | + | Originally from the Bastet server, I transfered to Tale 5 with some friends. (Now we form the Bastet Hounds Guild). |
| | My primary passion in Egypt is glassblowing and glassmaking. I also enjoy the Test of Pyrotechnics, Thought puzzles, and crossbreeding flowers. | | My primary passion in Egypt is glassblowing and glassmaking. I also enjoy the Test of Pyrotechnics, Thought puzzles, and crossbreeding flowers. |
| | I am always open to helping people in any way that I can, so please chat me anytime. | | I am always open to helping people in any way that I can, so please chat me anytime. |
| Line 21: |
Line 21: |
| | | | |
| | I am also willing to sell sheet glass, glass jars, glass pipes, glass rods, glass knives, glass torches. Bulk orders are subject to approval :) | | I am also willing to sell sheet glass, glass jars, glass pipes, glass rods, glass knives, glass torches. Bulk orders are subject to approval :) |
| | + | |
| | + | :For questions about getting started making glass, please see my guide: http://www.atitd.org/wiki/tale5/User:Avanya/Glassmaking_guide |
| | | | |
| | ==Buying== | | ==Buying== |
| Line 27: |
Line 29: |
| | This includes: | | This includes: |
| | *Mushrooms (Sand Spore, Slaves Bread, Razor's Edge, Peasant Foot, and Salt Water Fungus). | | *Mushrooms (Sand Spore, Slaves Bread, Razor's Edge, Peasant Foot, and Salt Water Fungus). |
| − | *Cobra Skins (as many as possible)
| |
| | | | |
| | I am also always happy to purchase | | I am also always happy to purchase |
| Line 36: |
Line 37: |
| | | | |
| | I am hoping to help Egypt make better use of its Portable Star Labs. I see so many of them laying around unused, or used as decorations. They are meant to be used and returned to a university of Art (preferably Sterope). I have a Law Petition circulating to help us use them more effectively. Please remember they are used for opening new Star recipes only...they are NOT part of the Principle or Test of Pyrotechnics!! They are given out in a limited amount, so they are a precious resource. Please see http://www.atitd.org/wiki/tale5/Portable_Star_Lab if you have any questions, or chat me in game. | | I am hoping to help Egypt make better use of its Portable Star Labs. I see so many of them laying around unused, or used as decorations. They are meant to be used and returned to a university of Art (preferably Sterope). I have a Law Petition circulating to help us use them more effectively. Please remember they are used for opening new Star recipes only...they are NOT part of the Principle or Test of Pyrotechnics!! They are given out in a limited amount, so they are a precious resource. Please see http://www.atitd.org/wiki/tale5/Portable_Star_Lab if you have any questions, or chat me in game. |
| | + | |
| | + | In addition, I am happy to help anyone with passing the principle of Pyrotechnics. If you have questions how to use a mortar, make stars, or anything pyro related, please chat me. If I can't help, I can probably direct you to someone that can! |
| | | | |
| | ==Mutagenics Research== | | ==Mutagenics Research== |
| | + | You can find my test data for mutagens at the following page: |
| | + | http://www.atitd.org/wiki/tale5/User:Avanya/mutagenics |
| | + | My most recent data is not on this page, because it got too long to put it all on there, lol. But if you are interested, please chat me, and I can get you copies of data from my notes. |
| | + | ==Test Ideas== |
| | + | [[Test of Gemology]] |
| | | | |
| − | I am trying to help characterize the mutagens that are being researched.
| + | [[Test of Sponsorship]] |
| − | Specially, I'm trying to determine which gene ranges are swapped from the left and right splints.
| |
| − | I won't bore you here with genetics theory, it is all explained much more clearly elsewhere on the wiki.
| |
| − | But I would like to make my data available to others that share this interest.
| |
| − | | |
| − | This is my first time doing this, so PLEASE chat me if you think I am doing something wrong. Right now, our guru's of genetics are not playing, so I don't have a good source to verify this with. I welcome all input!!
| |
| − | | |
| − | ===Fuzzy, Hairy, Spongy===
| |
| − | Tay 06 recipe, Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 33/Ava 34)
| |
| − | Observed:
| |
| − | :Left Spoint: 1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | :Right Splint: 2 weed/0 water/1 seed / 1 flax/ 0 rotten
| |
| − | | |
| − | Right splint was affected in (GYGYGY) gene, specifically NOT the middle GY genes.
| |
| − | Left splint had a junk gene swapped.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *RYY
| |
| − | *RGYG
| |
| − | *GGRG <-- that pinpoints it as the third Y in (GYGYGY) being replaced with a G
| |
| − | | |
| − | Right Genome: K(OR)GR(GYGYG<span style="border:1px solid green;">G</span>)GRGY(OR)YYRG(OR)YY(OR)RK
| |
| − | :gene targeted was 10/27.
| |
| − | :gene range is 9.5/27 to 10.5/27 or <span style="color:red;">35.185% to 38.889%</span>
| |
| − | :also reveals that a "Y" gene was moved to the left splint
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *KOR
| |
| − | *ORRK
| |
| − | *RGY
| |
| − | *YYOR
| |
| − | *ROOY
| |
| − | *ORYY
| |
| − | *GYY <--- proves it
| |
| − | :Looking for where a junk G was replaced with a Y gene, and no new genes were created. (Phenotype was not altered)
| |
| − | | |
| − | Left Genome: K(OR)GR(GYGYGY)<span style="border:1px solid green;">Y</span>RGY(OR)YYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 11/27
| |
| − | :gene range is 10.5/27 to 11.5/27 or <span style="color:red;">38.889% to 42.593%</span>
| |
| − | </div>
| |
| − | | |
| − | ===Calico, Mottle, Slimy ===
| |
| − | Tay 03 recipe, Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green (Ava 35, Ava 36)
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :Left Splint: 1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | :Right Splint: 1 weed/01 water/1 seed / 2 flax/ 0 rotten
| |
| − | | |
| − | Both splints had junk genes swapped.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *GGYG <-- that pinpoints it as 12th gene, R being replaced with G
| |
| − | | |
| − | Right Genome: K(OR)GR(GYGYG)G<span style="border:1px solid green;">G</span>)GY(OR)YYRG(OR)YY(OR)RK
| |
| − | :gene targeted was 12/27.
| |
| − | :gene range is 11.5/27 to 12.5/27 or <span style="color:red;">42.593% to 46.296%</span>
| |
| − | :also reveals that a "R" gene was moved to the left splint
| |
| − | | |
| − | Left Splint:
| |
| − | :Looking for where a junk G was replaced with a R gene, and no new genes were created. (Phenotype was not altered)
| |
| − | Solvents on Left Splint showed:
| |
| − | *ORGR
| |
| − | *GYGY
| |
| − | *YGYG
| |
| − | *YORY
| |
| − | *RYYR
| |
| − | *ROYY
| |
| − | *RGR
| |
| − | *GYOR
| |
| − | *RGYO
| |
| − | *YYO
| |
| − | *YGY
| |
| − | *RGYG
| |
| − | *YRR <--- confirms G --> R switch. Only two places it could be. 11th or 20th.
| |
| − | *RRRO <-- proves 20th gene was swapped to R.
| |
| − | | |
| − | | |
| − | Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYR<span style="border:1px solid green;">R</span>(RO)YY(OR)RK
| |
| − | :gene targeted was 20/27
| |
| − | :gene range is 19.5/27 to 20.5/27 or <span style="color:red;">72.222% to 75.926%</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Silken / Clarity (Ava 28, Ava 29)
| |
| − | :phenotypes: Silken bulb unchanged, Clarity had a cyan gene broken.
| |
| − | | |
| − | :Left splint: Silken Genome: KRRROOOYYYGGGUUUIIIK
| |
| − | :left target 72.222%-75.962% is 13/18 gene --> 1st U.
| |
| − | | |
| − | :Right splint: Clarity Genome: KIRIRIOIOIYIY(GORR)GRORGRRO(GORR)GRORGRROK
| |
| − | :right target 42.593%-46.296% is 16/36 gene --> 2nd R in the first cyan gene set (GORR)
| |
| − | :resultant right genome: KIRIRIOIOIYIY(GORR)GRORGRRO(GOR<span style="border:1px solid green;">U</span>)GRORGRROK
| |
| − | | |
| − | :we know now what left genome is, since it's U was replaced with an R from Clarity:
| |
| − | :resultant left genome: KRRROOOYYYGGG<span style="border:1px solid green;">R</span>UUIIIK
| |
| − | | |
| − | These results are compatible with observations!!
| |
| − | (not confirmed with solvents, just observation and TEST 1 results)
| |
| − | </div>
| |
| − | | |
| − | ===Calico Green Striped ===
| |
| − | Tay 02 recipe, Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :both new splints had same phenotypes. (Ava 37/Ava 38)
| |
| − | | |
| − | Both splints had junk genes swapped, or same type of gene swapped.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *RGRO
| |
| − | *GRGY
| |
| − | *ORYY
| |
| − | *ROYY
| |
| − | *GROY
| |
| − | *GRG
| |
| − | *GYOR
| |
| − | *YORY
| |
| − | *YGYG
| |
| − | no anomolies found yet. Standard Junk genes all covered
| |
| − | | |
| − | :Solvents on Left Splint showed:
| |
| − | *GRGY
| |
| − | *ORYY
| |
| − | *YGRG
| |
| − | *GROY
| |
| − | *RGRO
| |
| − | *ORGR
| |
| − | *GYGY
| |
| − | *YGYG
| |
| − | *GYOR
| |
| − | *YRG
| |
| − | *ORRK
| |
| − | | |
| − | no anomolies found, and standard junk genes all covered.
| |
| − | :this test is inconclusive
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Silken / Vampire (Ava 26, 27)
| |
| − | :phenotypes: Silken bulb unchanged, Vampire had center go magenta (ie had an IO get broken)
| |
| − | | |
| − | :Left splint: Silken Genome: KRRROOOYYYGGGUUUIIIK
| |
| − | :Right splint: Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K
| |
| − | :so far, no conclusions made
| |
| − | </div>
| |
| − | | |
| − | | |
| − | ===Crackly, Dry, Spongy ===
| |
| − | Tay 05 recipe, Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :both new splints had same phenotypes. (Ava 39/Ava 40)
| |
| − | | |
| − | Both splints had junk genes swapped, or same type of gene swapped.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *not tested yet
| |
| − | | |
| − | :Solvents on Left Splint showed:
| |
| − | *GYGR
| |
| − | *RYYR
| |
| − | *YORR
| |
| − | *GROY
| |
| − | *OYY
| |
| − | *YRG
| |
| − | *RGR
| |
| − | *GYOR
| |
| − | *RGYO
| |
| − | *GYG
| |
| − | *OYYO
| |
| − | *ROYY
| |
| − | *GRGY
| |
| − | | |
| − | no anomolies found, and standard junk genes all covered.
| |
| − | Seems that the <span style="color:red;">Same Gene type</span> was swapped between splints :(
| |
| − | | |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Blush / Morning (Ava 31, 32)
| |
| − | :phenotypes: Both phenotypes were unchanged
| |
| − | | |
| − | :Left splint: Blush Genome: KGRORVGOOOVUROOVIOVIOOVIOOOK
| |
| − | :Right splint: Morning: KIRRRIYYYIYYYUYYYUYYYURORURORGRRRGYYYGORRGRROK
| |
| − | :so far, no conclusions made
| |
| − | </div>
| |
| − | | |
| − | | |
| − | ===Dry, Prickly, Reticulated===
| |
| − | Tay 01 recipe, Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 41/Ava 42)
| |
| − | Observed:
| |
| − | :Left Spoint: 1 weed/1 water/1 seed/2 flax/<span style="color:red;">1 rotten flax</span>
| |
| − | :Right Splint: 0 zeed!!
| |
| − | | |
| − | Right splint had no gene groups broken, but gained an additional gene sequence of RRGY
| |
| − | Left splint had the (RO) gene group broken.
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *ORR
| |
| − | *GYG
| |
| − | *YRR <-- that pinpoints it as the third G directly after (GYGYGY) as being replaced with an R
| |
| − | | |
| − | Right Genome: K(OR)GR(GYGYGY)<span style="border:1px solid green;">R</span>RGY(OR)YYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 11/27.
| |
| − | :gene range is 10.5/27 to 11.5/27 or <span style="color:red;">38.889% to 42.593%</span>
| |
| − | :also reveals that a "G" gene was moved to the left splint
| |
| − | | |
| − | :Left Splint:
| |
| − | :The phenotype revealed that the (RO) seed gene was mutated.
| |
| − | :The right gene results show that an "R" gene was switched w/ a "G" gene.
| |
| − | | |
| − | Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(<span style="border:1px solid green;">G</span>O)YY(OR)RK
| |
| − | :gene targeted was 21/27
| |
| − | :gene range is 20.5/27 to 21.5/27 or <span style="color:red;">75.926% to 79.630%</span>
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Fracture / Crown (Ava 43,44)
| |
| − | :phenotypes: Fracture became giant, colors unchanged. Crown petals went from red to magenta
| |
| − | | |
| − | :Left splint: Fracture: K(ROYG)(ROY(G)YOR)GOR(GOOO)K
| |
| − | :Test 1 predicts the 1st Y in the "ROYGYOR" set will be swapped. if so, it should be a 1 giant + 1 dwarf = small size.
| |
| − | :observed: its a giant. no color changes
| |
| − | To be a size 1 giant, the both 1 giant and 1 dwarf gene must have been disabled.
| |
| − | This would be accomplished if the shared "G" were swapped.
| |
| − | This is just outside the predicted range of TEST 1. Perhaps I am not rounding out enough?
| |
| − | | |
| − | :Right splint: Crown: K(UROO)(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K
| |
| − | :Test 1 predicts that gene 29 will swap. (2nd R in the first (UROR) set. If so, a yellow gene will be broken.
| |
| − | :observed: the petals are more magenta colored than red. This is consistent w/ above prediction. It could also be a gene before or after this one that moved, and give the same results, so the exact gene position isn't certain w/o solvent testing.
| |
| − | | |
| − | Hypothesized Ava 43 genome:
| |
| − | :K(ROYG)ROY(<span style="border:1px solid green;">R</span>O)YORGOR(GOOO)K
| |
| − | gene 8/18 targeted.
| |
| − | 7.5/18 to 8.5/18 range is 41.667% to 47.222% for left splint target
| |
| − | | |
| − | Range overlap is <span style="color:red;">41.667% to 42.593%.</span>
| |
| − | </div>
| |
| − | | |
| − | Hypothesized Ava 44 genome:
| |
| − | :K(UROO)(UROO)(UROO)VURVOOVURVORV(URO<span style="border:1px solid green;">G</span>)(UROR)(UROR)K
| |
| − | no further range refinement possible w/o solvent testing.
| |
| − | | |
| − | </div>
| |
| − | | |
| − | ===Crackly, Prickly Spongy ===
| |
| − | Tay 04 recipe, Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :both new splints had same phenotypes. (Ava 49/Ava 50)
| |
| − | | |
| − | Both splints had junk genes swapped, or same type of gene swapped.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *not tested yet
| |
| − | | |
| − | :Solvents on Left Splint showed:
| |
| − | *KORG
| |
| − | *ORGR
| |
| − | *RYY
| |
| − | *YGY, GYG
| |
| − | *YORY
| |
| − | *GROY
| |
| − | *ORYY
| |
| − | *GYGY
| |
| − | *RGRO
| |
| − | *YYRG
| |
| − | *YRGR
| |
| − | *GYGR
| |
| − | *RGYO
| |
| − | | |
| − | no anomolies found, and standard junk genes all covered.
| |
| − | Seems that the <span style="color:red;">Same Gene type</span> was swapped between splints :(
| |
| − | further tests are needed to characterize this mutagen
| |
| − | | |
| − | </div>
| |
| − | | |
| − | ===Crackly, Hairy, Prickly===
| |
| − | Tay 07 recipe, Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 51/Ava 52)
| |
| − | Observed:
| |
| − | :Left Spoint: 2 weed/1 water/1 seed/2 flax/0 rotten had an extra weed!
| |
| − | :Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten not changed
| |
| − | | |
| − | Left splint was affected in (OR) gene.
| |
| − | Right splint had a junk gene swapped.
| |
| − | | |
| − | Solvents on Left Splint (Ava51) showed:
| |
| − | *YYOY <-- that pinpoints it as the last (OR) broken. R was replaced w/ Y.
| |
| − | :Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(O<span style="border:1px solid green;">Y</span>))RK
| |
| − | :gene targeted was 26/27
| |
| − | :gene range is 25.5/27 to 26.5/27 or <span style="color:red;">94.444% to 98.145%</span>
| |
| − | | |
| − | Right splint had a junk Y replaced.
| |
| − | Solvents on Right Splint showed:
| |
| − | *GRGY
| |
| − | *GROY
| |
| − | *GYGY
| |
| − | *RGRO
| |
| − | *RGRG
| |
| − | *RRK
| |
| − | *YRG
| |
| − | *YYO
| |
| − | *YOR
| |
| − | *ORG
| |
| − | *ORR <--- pinpoints it (after other solvents eliminated some possibly Y's).
| |
| − | The 17th Y gene is swapped out and was replaced with an R
| |
| − | :Right genome: K(OR)GR(GYGYGY)GRGY(OR)<span style="border:1px solid green;">R</span>YRG(RO)YY(OR))RK
| |
| − | :gene targeted was 17/27
| |
| − | :gene range is 16.5/27 to 17.5/27 or <span style="color:red;">61.111% to 64.815%</span>
| |
| − | </div>
| |
| − | | |
| − | ===Crackly, Phosphorescent, Slimy ===
| |
| − | Tay 08 recipe, Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 53/Ava 54)
| |
| − | Observed:
| |
| − | :Left Spoint: 0 weed/1 water/1 seed/0 flax/0 rotten ... no weed and no flax are different
| |
| − | :Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten... 1 extra weed step
| |
| − | | |
| − | Left splint was affected in ROY sequence (which is required to harvest flax, see flax genome theories for more info). However, it was not a *(RO) that was changed, or the seed count would have gone down. Furthermore, we need a new OR gene, to account for the 1 less weed step.
| |
| − | :looks like the 2nd to last Y should be switched to a R. ... YYRG(RO)RY(OR)RK. This would add (OR) and removed the (ROY).
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *GYG
| |
| − | *RGR
| |
| − | *ROR <-- that pinpoints it as the gene we suspected.
| |
| − | :the 22nd gene, Y, is replaced with an R
| |
| − | :gene range is 21.5/27 to 22.5/27 or <span style="color:red;">83.333% to 87.037%</span>
| |
| − | :Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(OR)<span style="border:1px solid green;">R</span>Y(OR)RK
| |
| − | | |
| − | Right splint lost an (-1 weed) gene, so (OR) was broken somewhere in the genome.
| |
| − | We know a R was replaced with a Y, from the left splint tests.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *ORY
| |
| − | *ROY
| |
| − | *GRGY
| |
| − | *GYGY
| |
| − | *YOR
| |
| − | *ROYY
| |
| − | *YYRG
| |
| − | *RGR
| |
| − | *OYYO
| |
| − | *YORY
| |
| − | *KOY <--- shows its the second gene, R, that is swapped with a Y from the other splint
| |
| − | :gene targeted was 2/27.
| |
| − | :gene range is 1.5/27 to 2.5/27 or <span style="color:red;">5.556% to 9.259%</span>
| |
| − | :Right Genome: K(O<span style="border:1px solid green;">Y</span>)GR(GYGYGY)GRGY(OR)YYRG(OR)YY(OR)RK
| |
| − | </div>
| |
| − | | |
| − | ===Calico Green Smelly ===
| |
| − | Tay 9 recipe, Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :both new splints had same phenotypes. (Ava 55/Ava 56)
| |
| − | | |
| − | Both splints had junk genes swapped, or same type of gene swapped.
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *GRG
| |
| − | *GRO
| |
| − | *GYGY
| |
| − | *YGYG
| |
| − | *ORYY
| |
| − | *ORGR
| |
| − | *YORY
| |
| − | *YYOR
| |
| − | *RGYO
| |
| − | *OYYO
| |
| − | *GYGR
| |
| − | *RYY
| |
| − | *YRGR
| |
| − | *ORRK
| |
| − | no anomolies found yet. Standard Junk genes all covered.
| |
| − | | |
| − | This mutation swapped same gene type between splints
| |
| − | :this test is inconclusive
| |
| − | </div>
| |
| − | | |
| − | ===Crackly, Spotted, Striped===
| |
| − | Tay 10 recipe, Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 57/Ava 58)
| |
| − | Observed:
| |
| − | :Left Spoint: 2 weed/0 water/1 seed/0 flax/0 rotten
| |
| − | :Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten
| |
| − | | |
| − | Left splint was affected in (GYGYGY) gene, specifically in the middle GY genes.
| |
| − | This breaks up both Flax genes (GYGY) as well as the water gene (GYGYGY), which would account for our observations.
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *YGRG <-- that pinpoints it as the middle Y in (GYGYGY) being replaced with a R
| |
| − | :Left Genome: K(OR)GR(GYG<span style="border:1px solid green;">R</span>GY)GRGY(OR)YYRG(OR)YY(OR)RK
| |
| − | :gene targeted was 8/27.
| |
| − | :gene range is 7.5/26 to 8.5/27 or <span style="color:red;">27.778% to 31.481%</span>
| |
| − | | |
| − | Right splint had a -1 weed gene broken (OR). From above, we know it was the R that was swapped with a Y from the Left splint.
| |
| − | Just have to identify which of the three (OR) genes was broken.
| |
| − | Solvents on Right Splint showed:
| |
| − | *RGRG
| |
| − | *RGY
| |
| − | *GYG
| |
| − | *YRGY
| |
| − | *RGY
| |
| − | *ORG
| |
| − | *YGY
| |
| − | *ORY
| |
| − | *RGY
| |
| − | *YOR
| |
| − | *YYR
| |
| − | *RRY
| |
| − | *GYO
| |
| − | *OYRB <--- pinpoints the (OR) gene at the end as switching to (OY).
| |
| − | | |
| − | Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(O<span style="border:1px solid green;">Y</span>)RK
| |
| − | :gene targeted was 26/27
| |
| − | :gene range is 25.5/27 to 26.5/27 or <span style="color:red;">94.444% to 98.148%</span>
| |
| − | </div>
| |
| − | | |
| − | ===Phosphorescent, Slimy, Smelly ===
| |
| − | Tay 11 recipe, Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 59/Ava 60)
| |
| − | Observed:
| |
| − | :Left Spoint: same phenotype
| |
| − | :Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten... 1 extra weed step
| |
| − | | |
| − | Right splint had an extra weeding step. This means an (OR) gene was broken up.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *YRRR <-- that pinpoints it as the last O gene --> R gene.
| |
| − | :the 25nd gene, O, is replaced with an R
| |
| − | :gene range is 24.5/27 to 25.5/27 or <span style="color:red;">90.741% to 94.444%</span>
| |
| − | :Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(<span style="border:1px solid green;">R</span>R)RK
| |
| − | | |
| − | Left splint lost an R gene in the junk genes somewhere, which was replaced with an O gene.
| |
| − | Solvents on Left Splint showed:
| |
| − | *YGRG
| |
| − | *OGRO <--- shows its the 19th gene, R, that is swapped with a G from the other splint.
| |
| − | :gene targeted was 19/27.
| |
| − | :gene range is 18.5/27 to 19.5/27 or <span style="color:red;">68.519% to 72.222%</span>
| |
| − | :Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YY<span style="border:1px solid green;">O</span>G(RO)YY(OR)RK
| |
| − | </div>
| |
| − | | |
| − | ==Crosses for Lily Requirements==
| |
| − | | |
| − | ===Aiming for 75% Green petals ===
| |
| − | :Stragety: Add cyan to one set of inner petals on Morning, then break one of the two yellow genes for that same set. This will turn that inner set of petals green. The outer two sets of petals are already green, so that will be 3/4 green.
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | CROSS 1: Morning / Crown with calico,dry, spotted (Sterope 01)
| |
| − | Aiming to swap first genes of both splints.
| |
| − | Unmutated Morning:
| |
| − | : K(IRRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K
| |
| − | : Cyan1OSMSIS(IRRR)+Yel2OSMSIS(IYYY)+Yel2IEP(UYYY)+Yel2IWP(UROR)+Cyan1OSP(GRRR)+Yel1OSP(GYYY)+Cyan1ONP(GORR)+Yel1ONP(GRRO)
| |
| − | Unmutated Crown:
| |
| − | : K(UROO)(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K
| |
| − | : Red3=[Mag3IWP(UROO)+Ye3IWP(UROR)] + 5V
| |
| − | | |
| − | :This mutagen will Turn (IRRR) (cyan on stamen) into (URRR), cyan on Inner East Petal.
| |
| − | | |
| − | Applied mutagen. (Ava 45/Ava46)
| |
| − | Observed:
| |
| − | :Left Spoint(Ava 45): Inner east petals now greenish yellow. <span style="color:red;">the cyan add was a success!!</span>
| |
| − | :Genome of Ava 45: K(<span style="border:1px solid green;">U</span>RRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K
| |
| − | | |
| − | :Right Splint(Ava 46): Inner petals look orange, not red
| |
| − | :Genome of Ava 46: K<span style="border:1px solid green;">I</span>ROO(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K
| |
| − | Right splint had first gene broken, UROO (magenta inner petals) --> IROO (junk).
| |
| − | inner petal set now 3 yellow shades + 2 magenta shades = red + red + yellow, or a dark orange
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | CROSS 2: Ava45 / Blush with Fuzzy, Hairy, Spongy (Tay 06)
| |
| − | : aiming to break up a (UYYY) inner yellow gene on Ava 45
| |
| − | Mutation should swap gene 18/44 (Y) on Ava 45 with gene 10/26 (V) blush.
| |
| − | Unmutated Ava 45: K(URRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K
| |
| − | Unmutated Blush: K(GROR)V(GOOO)V(UROO)V(IO)V(IOO)V(IOOO)K
| |
| − | | |
| − | Ava 47 / 48 resulted.
| |
| − | Observed:
| |
| − | left splint (Ava47) does have predicted colors. Green on outside petal sets. one green and one yellow inner petal sets.
| |
| − | right splint (Ava48): colors appear to be blush. No observed change.
| |
| − | | |
| − | Hypothesized Ava 47 genome:
| |
| − | :K(URRR)(IYYY)(IYYY)(UYYY)UY<span style="border:1px solid green;">V</span>Y(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K
| |
| − | Hypothesized Ava 48 Genome:
| |
| − | : K(GROR)V(GOOO)<span style="border:1px solid green;">Y</span>(UROO)V(IO)V(IOO)V(IOOO)K
| |
| | | | |
| − | both are consistent with expected and observed results
| + | [[Test of Magnificent Gardens]] |
| − | </div>
| |
