User:Avanya

Greetings!

Originally from the Bastet server, I transfered to Tale 5 only a few months ago with some friends. (Now we form the Bastet Hounds Guild). My primary passion in Egypt is glassblowing and glassmaking. I also enjoy the Test of Pyrotechnics, Thought puzzles, and crossbreeding flowers. I am always open to helping people in any way that I can, so please chat me anytime. I reside in Alcyone, at 1748, 2478. It's a short jog north of the chariot. My thought puzzle and Art coordinates can be found in my /info.

Glass Services

I sell most glass products, but I do not use a trading point system. I prefer to work out a trade that is fair and considered what the buyer has available to trade. My products include:

• Thermometers
• Barometers (5k, 6k quality)
• Hookah bowls (will assemble into finished hookah with your 14 canvas)
• Chemistry Calibration kits
• Distillation Coils 7.5k quality
• Wine glasses (sorry 8k quality and down, I need more practice)
• Mirrors (with your 200 silver powder though)

I am also willing to sell sheet glass, glass jars, glass pipes, glass rods, glass knives, glass torches. Bulk orders are subject to approval :)

Buying

I am currently buying materials needed to continue my work with characterizing mutagens. This includes:

• Mushrooms (Sand Spore, Slaves Bread, Razor's Edge, Peasant Foot, and Salt Water Fungus).
• Cobra Skins (as many as possible)

I am also always happy to purchase

• Silk, Sea Lily Bulbs, and Dung.

Yes Dung. I love Dung. (Test of Pyro should be renamed Test of Tons-of-Dung)

Star Labs

I am hoping to help Egypt make better use of its Portable Star Labs. I see so many of them laying around unused, or used as decorations. They are meant to be used and returned to a university of Art (preferably Sterope). I have a Law Petition circulating to help us use them more effectively. Please remember they are used for opening new Star recipes only...they are NOT part of the Principle or Test of Pyrotechnics!! They are given out in a limited amount, so they are a precious resource. Please see http://www.atitd.org/wiki/tale5/Portable_Star_Lab if you have any questions, or chat me in game.

Mutagenics Research

I am trying to help characterize the mutagens that are being researched. Specially, I'm trying to determine which gene ranges are swapped from the left and right splints. I won't bore you here with genetics theory, it is all explained much more clearly elsewhere on the wiki. But I would like to make my data available to others that share this interest.

This is my first time doing this, so PLEASE chat me if you think I am doing something wrong. Right now, our guru's of genetics are not playing, so I don't have a good source to verify this with. I welcome all input!!

Fuzzy, Hairy, Spongy

Tay 06 recipe, Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 33/Ava 34) Observed:

Left Spoint: 1 weed/1 water/1 seed/2 flax/0 rotten

Right Splint: 2 weed/0 water/1 seed / 1 flax/ 0 rotten

Right splint was affected in (GYGYGY) gene, specifically NOT the middle GY genes. Left splint had a junk gene swapped.

Solvents on Right Splint showed:

• RYY
• RGYG
• GGRG <-- that pinpoints it as the third Y in (GYGYGY) being replaced with a G

Right Genome: K(OR)GR(GYGYGG)GRGY(OR)YYRG(OR)YY(OR)RK

gene targeted was 10/26.

gene range is 9.5/26 to 10.5/26 or 35.185% to 38.889%

also reveals that a "Y" gene was moved to the left splint

Solvents on Left Splint showed:

• KOR
• ORRK
• RGY
• YYOR
• ROOY
• ORYY
• GYY <--- proves it

Looking for where a junk G was replaced with a Y gene, and no new genes were created. (Phenotype was not altered)

Left Genome: K(OR)GR(GYGYGY)YRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 11/27

gene range is 10.5/27 to 11.5/27 or 38.889% to 42.593%

Calico, Mottle, Slimy

Tay 03 recipe, Renard opened

TEST 1: Nile Green / Nile Green (Ava 35, Ava 36) Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

Left Splint: 1 weed/1 water/1 seed/2 flax/0 rotten

Right Splint: 1 weed/01 water/1 seed / 2 flax/ 0 rotten

Both splints had junk genes swapped.

Solvents on Right Splint showed:

• GGYG <-- that pinpoints it as 12th gene, R being replaced with G

Right Genome: K(OR)GR(GYGYG)GG)GY(OR)YYRG(OR)YY(OR)RK

gene targeted was 12/26.

gene range is 11.5/26 to 12.5/26 or 42.593% to 46.296%

also reveals that a "R" gene was moved to the left splint

Left Splint:

Looking for where a junk G was replaced with a R gene, and no new genes were created. (Phenotype was not altered)

Solvents on Left Splint showed:

• ORGR
• GYGY
• YGYG
• YORY
• RYYR
• ROYY
• RGR
• GYOR
• RGYO
• YYO
• YGY
• RGYG
• YRR <--- confirms G --> R switch. Only two places it could be. 11th or 20th.
• RRRO <-- proves 20th gene was swapped to R.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRR(RO)YY(OR)RK

gene targeted was 20/27

gene range is 19.5/27 to 20.5/27 or 72.222% to 75.926%

TEST 2: Silken / Clarity (Ava 28, Ava 29)

phenotypes: Silken bulb unchanged, Clarity had a cyan gene broken.

Left splint: Silken Genome: KRRROOOYYYGGGUUUIIIK

left target 72.222%-75.962% is 13/18 gene --> 1st U.

Right splint: Clarity Genome: KIRIRIOIOIYIY(GORR)GRORGRRO(GORR)GRORGRROK

right target 42.593%-46.296% is 16/36 gene --> 2nd R in the first cyan gene set (GORR)

resultant right genome: KIRIRIOIOIYIY(GORR)GRORGRRO(GORU)GRORGRROK

we know now what left genome is, since it's U was replaced with an R from Clarity:

resultant left genome: KRRROOOYYYGGGRUUIIIK

These results are compatible with observations!! (not confirmed with solvents, just observation and TEST 1 results)

Calico Green Striped

Tay 02 recipe, Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 37/Ava 38)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Right Splint showed:

• RGRO
• GRGY
• ORYY
• ROYY
• GROY
• GRG
• GYOR
• YORY
• YGYG

no anomolies found yet. Standard Junk genes all covered

Solvents on Left Splint showed:

• GRGY
• ORYY
• YGRG
• GROY
• RGRO
• ORGR
• GYGY
• YGYG
• GYOR
• YRG
• ORRK

no anomolies found, and standard junk genes all covered.

this test is inconclusive

TEST 2: Silken / Vampire (Ava 26, 27)

phenotypes: Silken bulb unchanged, Vampire had center go magenta (ie had an IO get broken)

Left splint: Silken Genome: KRRROOOYYYGGGUUUIIIK

Right splint: Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K

so far, no conclusions made

Crackly, Dry, Spongy

Tay 05 recipe, Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 39/Ava 40)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Right Splint showed:

• not tested yet

Solvents on Left Splint showed:

• GYGR
• RYYR
• YORR
• GROY
• OYY
• YRG
• RGR
• GYOR
• RGYO
• GYG
• OYYO
• ROYY
• GRGY

no anomolies found, and standard junk genes all covered. Seems that the Same Gene type was swapped between splints :(

TEST 2: Blush / Morning (Ava 31, 32)

phenotypes: Both phenotypes were unchanged

Left splint: Blush Genome: KGRORVGOOOVUROOVIOVIOOVIOOOK

Right splint: Morning: KIRRRIYYYIYYYUYYYUYYYURORURORGRRRGYYYGORRGRROK

so far, no conclusions made

Dry, Prickly, Reticulated

Tay 01 recipe, Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 41/Ava 42) Observed:

Left Spoint: 1 weed/1 water/1 seed/2 flax/1 rotten flax

Right Splint: 0 zeed!!

Right splint had no gene groups broken, but gained an additional gene sequence of RRGY Left splint had the (RO) gene group broken.

Solvents on Left Splint showed:

• ORR
• GYG
• YRR <-- that pinpoints it as the third G directly after (GYGYGY) as being replaced with an R

Right Genome: K(OR)GR(GYGYGY)RRGY(OR)YYRG(OR)YY(OR)RK

gene targeted was 11/26.

gene range is 10.5/26 to 11.5/26 or 38.889% to 42.593%

also reveals that a "G" gene was moved to the left splint

Left Splint:

The phenotype revealed that the (RO) seed gene was mutated.

The right gene results show that an "R" gene was switched w/ a "G" gene.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(GO)YY(OR)RK

gene targeted was 21/27

gene range is 20.5/27 to 21.5/27 or 75.926% to 79.630%

TEST 2: Fracture / Crown (Ava 43,44)

phenotypes: Fracture became giant, colors unchanged. Crown petals went from red to magenta

Left splint: Fracture: K(ROYG)(ROY(G)YOR)GOR(GOOO)K

Test 1 predicts the 1st Y in the "ROYGYOR" set will be swapped. if so, it should be a 1 giant + 1 dwarf = small size.

observed: its a giant. no color changes

To be a size 1 giant, the both 1 giant and 1 dwarf gene must have been disabled. This would be accomplished if the shared "G" were swapped. This is just outside the predicted range of TEST 1. Perhaps I am not rounding out enough?

Right splint: Crown: K(UROO)(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K

Test 1 predicts that gene 29 will swap. (2nd R in the first (UROR) set. If so, a yellow gene will be broken.

observed: the petals are more magenta colored than red. This is consistent w/ above prediction. It could also be a gene before or after this one that moved, and give the same results, so the exact gene position isn't certain w/o solvent testing.

Hypothesized Ava 43 genome:

K(ROYG)ROY(RO)YORGOR(GOOO)K

gene 8/18 targeted. 7.5/18 to 8.5/18 range is 41.667% to 47.222% for left splint target

Range overlap is 41.667% to 42.593%.

Hypothesized Ava 44 genome:

K(UROO)(UROO)(UROO)VURVOOVURVORV(UROG)(UROR)(UROR)K

no further range refinement possible w/o solvent testing.

Crackly, Prickly Spongy

Tay 04 recipe, Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 49/Ava 50)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Right Splint showed:

• not tested yet

Solvents on Left Splint showed:

• KORG
• ORGR
• RYY
• YGY, GYG
• YORY
• GROY
• ORYY
• GYGY
• RGRO
• YYRG
• YRGR
• GYGR
• RGYO

no anomolies found, and standard junk genes all covered. Seems that the Same Gene type was swapped between splints :( further tests are needed to characterize this mutagen

Crosses for Lily Requirements

Aiming for 75% Green petals

Stragety: Add cyan to one set of inner petals on Morning, then break one of the two yellow genes for that same set. This will turn that inner set of petals green. The outer two sets of petals are already green, so that will be 3/4 green.

CROSS 1: Morning / Crown with calico,dry, spotted (Sterope 01) Aiming to swap first genes of both splints. Unmutated Morning:

K(IRRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K

Cyan1OSMSIS(IRRR)+Yel2OSMSIS(IYYY)+Yel2IEP(UYYY)+Yel2IWP(UROR)+Cyan1OSP(GRRR)+Yel1OSP(GYYY)+Cyan1ONP(GORR)+Yel1ONP(GRRO)

Unmutated Crown:

K(UROO)(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K

Red3=[Mag3IWP(UROO)+Ye3IWP(UROR)] + 5V

This mutagen will Turn (IRRR) (cyan on stamen) into (URRR), cyan on Inner East Petal.

Applied mutagen. (Ava 45/Ava46) Observed:

Left Spoint(Ava 45): Inner east petals now greenish yellow. the cyan add was a success!!

Genome of Ava 45: K(URRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K

Right Splint(Ava 46): Inner petals look orange, not red

Genome of Ava 46: KIROO(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K

Right splint had first gene broken, UROO (magenta inner petals) --> IROO (junk). inner petal set now 3 yellow shades + 2 magenta shades = red + red + yellow, or a dark orange

CROSS 2: Ava45 / Blush with Fuzzy, Hairy, Spongy (Tay 06)

aiming to break up a (UYYY) inner yellow gene on Ava 45

Mutation should swap gene 18/44 (Y) on Ava 45 with gene 10/26 (V) blush. Unmutated Ava 45: K(URRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K Unmutated Blush: K(GROR)V(GOOO)V(UROO)V(IO)V(IOO)V(IOOO)K

Ava 47 / 48 resulted. Observed: left splint (Ava47) does have predicted colors. Green on outside petal sets. one green and one yellow inner petal sets. right splint (Ava48): colors appear to be blush. No observed change.

Hypothesized Ava 47 genome:

K(URRR)(IYYY)(IYYY)(UYYY)UYVY(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K

Hypothesized Ava 48 Genome:

K(GROR)V(GOOO)Y(UROO)V(IO)V(IOO)V(IOOO)K

both are consistent with expected and observed results