Mutagens

See also: Genetics for Dummies

Mutagenics is researchable after Advanced Chemistry, Advanced Glassblowing, Viticulture and Gardening -> Crossbreeding, Herbiculture. (See the Talk:Mutagens page for the full path.)

What is a mutagen?

A mutagen works on two splints in a greenhouse. It switches a gene of the right first splint's genetic code with a gene of the left splint's genetic code. More precisely, it switches a gene located after the first n% of the genetic code of the first splint with one located after the first m% of the genetic code of the second splint. EG: If the genetic code of both splints is 150 genes long, and a mutagen switches the 12%th gene with the 34%th gene, then gene 18 (12*150/100) will switch with gene 51 (34*150/100).

Missing Mutagen Moss Alerts

A mutagenic moss must have all the positive attributes listed but cannot have any of the three negative attributes listed. Besides must having all of the positive attributes, it can also have any other attributes that are not one of the three negatives.

Example: Positives: Hairy, Mottled, Striped Negatives: Calico, Spongy, Spotted A correct moss could be Dry, Hairy, Mottled, Striped or Hairy, Fuzzy, Mottled, Reticulated, Striped. A wrong moss would be Hairy, Mottled (it is missing Striped) or Hairy, Mottled, Spotted, Striped (it has a negative Spotted in it).

Saqqarah Positives: Crackly, Slimy, Smelly  Negatives: Fuzzy, Hairy, Mottled  Contact Ariella

Using a Mutagen

• A mutagen is applied to the left and right splint plants in a greenhouse.
• The mutagen will target a gene on the left splint plant and a gene on the right splint plant and switch them.
• Two child plants are produced. The child plant with the lowest number is the modified left splint plant, the child plant with the highest number is the modified right splint plant.

Example: (See Genomes_and_Phenome_Theories_Links for genome info)

• In the greenhouse, put Vampire Sea Lily in left splint, Fracture Sea Lily in right splint.
• Apply imaginary mutagen crackly,spongy that theoretically switches the two genes which are in bolded type.
  

Left Splint--> IYIYIOIYIOIO (Vampire genome)
Right Splint--> ROYGROYGYORGORGOOO (Fracture genome)

• Two child bulbs result.
  

Avatar #112 IGIYIOIYIOIO
Avatar #113 ROYGROYGYORYORGOOO

Mutagen Recipes In Progress

Region	# Moss att	Moss Quantity	Positive Moss att	Negative Moss att	# Shroom Types	Recipe Shrooms	Shroom Bulk	Avatar	Lab coords
Meroe	#	#	+	-	~	#	x	#
Saqqarah	3		Crackly, Slimy, Smelly	Fuzzy, Hairy, Mottled				Ariella

Mutagens Discovered

Basic Mutagen info

When applying a mutagen to a plant that is not listed in a subsequent table you can calculate the targeted genes with the left and right target min. and max. percentages applied to the genome lengths (K.s not included).

  Note: substitute 100% by 99.99% to make formulas in the example below work all the time

Example 1 (adventurous use and target % determination)

• we put a plant with length 30 genome in left splint
• we put a plant with length 20 genome in the right splint
• we apply a mutagen with left percentages 10.02-11.52 and right percentages 53.11-55.30
• targeted gene calculation
  • left splint lowest possible impact 10.02 * 30 / 100 + 1 = 4.006, truncated --> 4
  • left splint highest possible impact 11.52 * 30 / 100 + 1 = 4.456, truncated --> 4
  • right splint lowest possible impact 53.11 * 20 / 100 + 1 = 11.622, truncated --> 11
  • right splint highest possible impact 55.30 * 20 / 100 + 1 = 12.060, truncated --> 12
• so the mutagen will target the 4th gene of the left splint plant and the 11th or 12th gene of the right splint plant (looking at the numbers shows a higher probability to hit the 11th)
• we proceed with the experiment and hope to hit the 11th
• we are lucky and hit the 11th !!!
• this means we can narrow down the right target zone max. % as follows: 11 * 100 / 20 = 55.00 (round down for min. %, round up for max. %)

Example 2 (planning for a correct hit)

• we want to hit (= replace) the 7th gene of a length 27 genome
• target min. % = (7-1) * 100 / 27 = 22.222
• target max. % = 7 * 100 / 27 = 25.926
• we look at the mutagen table and sort the table on Left target min. %
• mutagens with Left min. % >= 22.23 and Left max. % <= 25.92 are candidates to be goodies
• for each goodie, look at the possibilities of with the Right target values, you will likely find a plant with the genome you want in the perfect spot, especially when using short genomes
• if no goodies found yet (or if you are brave enough to look for other solutions) look at the mutagen table and sort the table on Right target min. %
• mutagens with Right min. % >= 22.23 and Right max. % <= 25.92 are candidates to be goodies
• for each goodie, look at the possibilities of with the Left target values, you will likely find a plant with the genome you want in the perfect spot, especially when using short genomes

  Feel free to contact Ariella or Pascalito with questions and with data updates you don't dare to apply or hesitate about -- accurate data is of EXTREME importance

Known Recipes

Mutagen Name	Recipe	Negative Moss att	Avatar	Region	Left splint target zone min. %	Left splint target zone max. %	Right splint target zone min. %	Right splint target zone max. %
Mottled, Phosphorescent and Reticulated	5 Mottled, Phosphorescent, Reticulated; 6 Acorn's Cap, 7 Dueling Serpents, 1 Dung Rot, 5 Eye of Osiris, 1 Hairy Tooth, 1 Heaven's Torrent	Prickly, Slimy, Spotted	Ariella	Saqqarah 1	0%	33.34%	33.33%	66.67%
Calico, Reticulated and Spotted	5 Calico, Reticulated, Spotted; 6 Bleeding Hand	Crackly, Mottled, Phosphorescent	Ariella	Saqqarah 2	0.00%	33.34%	91.66%	99.99%
Hairy, Mottled and Striped	6 Hairy, Mottled, Striped; 4 Brain, 1 Cobra Hood, 7 Earth Light, 4 Fish Hook, 5 Iron Knot, 1 Toad Skin	Calico, Spongy, Spotted	Eldrad/Ariella	Saqqarah 3	L-%	L+%	R-%	R+%
Mottled, Smelly and Spotted	3 Mottled, Smelly, Spotted; 3 Spiderlings	Dry, Phosphorescent, Spongy	Ariella	Saqqarah 4	L-%	L+%	R-%	R+%

Practical Tables

The following tables show which gene is hit by a mutagen on each splint side. Gene colors indicated with a CAPITAL character have been actually seen, those in lowercase have just been calculated based on the hit zone percentages.

Practical Sea Lily table

These tables show which gene is substituted, along with gene number (if available) counting genes from left to right starting at 1 just after the K.

L = left, R = right, numbers designate the targeted gene.
Genome lengths: Bl 26, Cl 36, Cr 29, De 16, Du 73, En 42, Fr 18, Mo 44, Si 18, Va 12

Mutagen Name	Bl L	Cl L	Cr L	De L	Du L	En L	Fr L	Mo L	Si L	Va L	Bl R	Cl R	Cr R	De R	Du R	En R	Fr R	Mo R	Si R	Va R
template row	Bl	Cl	Cr	De	Du	En	Fr	Mo	Si	Va	Bl	Cl	Cr	De	Du	En	Fr	Mo	Si	Va

Practical Orchid table

L = left, R = right, numbers designate the targeted gene.
Genome lengths: AN 30, Sa 28, SL 31, HD 34

Mutagen Name	AN L	Sa L	SL L	HD L	AN R	Sa R	SL R	HD R
template row	AN	Sa	SL	HD	AN	Sa	SL	HD

Practical Rose of Ra table

L = left, R = right, numbers designate the targeted gene.
Genome lengths: Bl 8, Go 12 HB 24, HoD ?, NB 45, OS 25, Pa 16, PG, 17, RD ?, WG 12

Mutagen Name	Bl L	Go L	HB L	HoD L	NB L	OS L	Pa L	PG L	RD L	WG L	Bl R	Go R	HB R	HoD R	NB R	OS R	Pa R	PG R	RD R	WG R
template row	Bl	Go	HB	HoD	NB	OS	Pa	PG	RD	WG	Bl	Go	HB	HoD	NB	OS	Pa	PG	RD	WG

Practical Sand Bloom table

L = left, R = right, numbers designate the targeted gene.
Genome lengths: Ca 34, Co 26, LD 18, LT 19, PM 29, Pr 23, Sa 24, Su 13

Mutagen Name	Ca L	Co L	LD L	LT L	PM L	Pr L	Sa L	Su L	Ca R	Co R	LD R	LT R	PM R	Pr R	Sa R	Su R
template row	Ca	Co	LD	LT	PM	Pr	Sa	Su	Ca	Co	LD	LT	PM	Pr	Sa	Su

Practical Flax table

L = left, R = right, numbers designate the targeted gene.
Genome lengths: NG 27, OE 39

Mutagen Name	MG L	OE L	MG R	OE R
template row	NG	OE	NG	OE

Practical Wheat table

L = left, R = right, numbers designate the targeted gene.
Genome lengths: Ab 19, Dr 14, Ma ?, Sa 22, Wi 19

Mutagen Name	Ab L	Dr L	Ma L	Sa L	Wi L	Ab R	Dr R	Ma R	Sa R	Wi R
template row	Ab	Dr	Ma	Sa	Wi	Ab	Dr	Ma	Sa	Wi

Practical Grape Vine table

L = left, R = right, numbers designate the targeted gene.
Genome lengths: Am 31, Ap 12, Ba 31, Co 18, Di ?, Fr 27?, Wi 25?

Mutagen Name	Am L	Ap L	Ba L	Co L	Di L	Fr L	Wi L	Am R	Ap R	Ba R	Co R	Di R	Fr R	Wi R
Mottled, Phosphorescent and Reticulated	R	Y	G	Y	G	O	O	G	Y	Y	Y	G	G	O
template row	Am	Ap	Ba	Co	Di	Fr	Wi	Am	Ap	Ba	Co	Di	Fr	Wi