OE05

Crackly, Prickly, Reticulated

This page serves as a repository of research notes on Toxin OE05, the fifth mutagen unlocked in Old Egypt by Renard. It uses 3 Crackly, Prickly, Reticulated (Negative to Hairy, Slimy, Spotted) moss and 7 Beehive, 2 Heart of Ash, 1 Iron Knot, 2 Toad Skin mushrooms.

First mutation test: L-Vampire R-Silken

No change to either flower.

Second mutation test: L-Silken R-Vampire

A magenta on the outer stamen has become Cyan. Hence an R was transferred from Silken, so the left splint affects a gene far on the left.

Discussion and next step

Since the right splint affects a gene so far to the left, in the first test either an I or a Y was swapped between the lilies. Either the right splint hits to the far right, or just left of center.

Clarity Fracture next.

Clarity: IRIRIOIOIYIYGORRGRORGRROGORRGRORGRRO

Fracture: ROYGROYGYORGORGOOO

Third mutation test: L-Clarity R-Fracture

Clarity's outer stamen has lost a cyan, Fracture's petals have lost color.

Clearly an I or R has been taken from Clarity and placed onto the very right-hand side of Fracture. However, it was not an R transferred. If it had taken the place of the G a GORR would have been formed, which would have put Cyan on the outer North petals. It could not have replaced one of the Os since that would have created a color visible on Clarity's outer stamen. Hence, an I must have been transferred, and it must have taken the place of the very last gene of Fracture since otherwise color would be visible on Fracture's stamen.

Right-Fracture 18(O)

Fourth mutation test: L-Fracture R-Clarity

Fracture is now Semi-Dwarf, so one of the giant genes has been modified. Clarity has lost Yellow in the Outer-North petals.

It was not an R transferred from fracture, because that would have either caused no change or made a visible change to the south petals Cyan. It could have been an O transferred and affecting gene 35, and it could have been a Y affecting anything. Further analysis might narrow it down.

Discussion and next test

We know from the results above that on the right splint, the following genes are affected:
Right-Silken 18(I)
Right-Vampire 12(O)

Given the results of the first test, an I was transferred from Vampire. The second test limits the possible range to the first two genes, so:
Left-Vampire 1(I)

That limits the genes affected on Silken and Fracture to be the first two.

As established in the fourth test, it was not an R transferred from fracture, so:
Left-Fracture 2(O) Left-Silken 2(R)

We know an I was transferred from Clarity on the left, so:
Left-Clarity 3(I)

And as discussed in the fourth test:
Right-Clarity 35(R)

Crimson and Morning both have stamen colors on their left, which make them good candidates for testing in that range.

Crimson: GYORIOOOIOO
Left splint: 6-9

Morning: IRRR
Left-splint: 3-4

Morning: GRRO
Right-splint: 42-43

Dusk: GORR UOOO
Right splint: 69-71

L-Crimson, R-Morning will not be particularly informative on the right splint, but results on the left splint should prove useful.

L-Morning, R-Dusk will transfer an R to dusk, and each result has a unique interpretation since UROO is a valid allele. If both petals remain unchanged, I won't know exactly where Morning was affected on the Left splint, but that's a risk I'm willing to take.

Fifth mutation test: L-Crimson, R-Morning

On the left, the only the innermost stamen of Crimson has lost color. On the right, north petals have lost Yellow on Morning, no change to the stamen.
Left-Crimson 8(O)

Sixth mutation test: L-Morning, R-Dusk

On the left, Morning is missing color only from it's innermost stamen. On the right, Dusk's inner-eastern petals have lost Magenta, but no color is present on the inner-west petals. Left-Morning 4(R) Right-Dusk 70(U)

Discussion and next step

The right splint is still ambiguous for Morning, so further testing will be required to narrow that down. The right splint of Crimson is now greatly limited:

Crimson (Final eight genes): GRROGRRO Right-splint: 101-102

On the left splint, only Energy (Which I don't care about testing) and Dusk still have any ambiguity.

Dusk: GROR GORR Left-splint: 5-6

Left-Dusk, Right-Crimson will be informative at least for the left splint. If a G is transferred to the right splint I won't know exactly what gene was affected there, but it can be cleared up through further testing quite easily.

Seventh mutation test: L-Dusk, R-Crimson

Cyan was lost from the outer-north petals, but magenta was not added to the south. Left-Dusk 5(G)

Discussion and next step

The left splint is now fully characterized. To really see what is going on in the right splint, one easy thing to do would be to add an R to it. Either there will be no change to either flower, or the GRRO allele will become a GRRR and add cyan to the outer-south petals.

Eighth mutation test: L-Morning, R-Crimson

South petals added Magenta.
Right-Crimson 102(O)

Ninth mutation test: L-Clarity, R-Morning

Cyan added to outer stamen.
Right-Morning 42(R)